Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Nov 21;215(4):531–542. doi: 10.1083/jcb.201607099

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2016 Yin et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 4. — Increased vulnerability to energy deprivation and altered mitochondrial transport in optic nerves from P₀-CNS mice. (A) Recordings of CAPs in 1-mo-old P₀-CNS mice (orange circles, n = 4) showed a rapid loss of CAPs and reduced recovery after 30 min of OGD compared with age-matched controls (green circles, n = 6). The inset bar graph summarizes recovery at the end of 2 h after OGD exposure. *, P = 0.026. (B) ATP levels were reduced in optic nerves from 1-mo-old P₀-CNS mice compared with controls. ATP levels were corrected to protein and normalized to WT controls. *, P = 0.015. (C–F) In organotypic slice cultures, mitochondrial transport was altered in myelinated P₀-CNS axons. (C and D) Node locations were identified after time-lapse imaging by immunostaining for Caspr (green) and registered to axonal Mito-Dendra2 labeling (red). Live time-lapse images of mitochondria (C′ and D′) are converted to kymographs (C″ and D″), where cessation of mitochondrial transport is frequently observed in P₀-CNS axons (P₀; D″, arrowheads). Bars: (horizontal) 10 µm; (vertical on kymograph) 100 s. (E) The mean speed of motile mitochondria was significantly reduced in P₀-CNS axons compared with WT axons over the entire axon segment studied (All) and within internodal (Inter) and nodal–paranodal axoplasm (N-PN). The mean mitochondrial speed in P₀-CNS axons was significantly reduced in nodal–paranodal axoplasm compared with internodal axoplasm. Bars and lines are mean + SEM. n = 27, 28, 23, 27, 22, and 24. (F) Mitochondrial stopping events were quantified in proximal (Prox) and distal (Dis) regions of the nodes. Stopping events were more common in the proximal juxtaparanode in WT axons and in the distal juxtaparanodal axoplasm in P₀-CNS axons. Bars with boxes and lines are median, interquartile range, and maximum/minimum. *, P < 0.05; ***, P < 0.001; t test; n = 32 and 23 (F) axons from three WT and three P₀-CNS explants.