Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Nov 21;215(4):499–513. doi: 10.1083/jcb.201608123

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2016 Thomas and Fromme

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 2. — Ypt31/32 and Ypt1 share an active site in TRAPPII. (A, left) Schematic depicting two possible mechanisms for TRAPPII-mediated Ypt31/32 activation. (right) The indicated rTRAPPI active site mutants were tested for their ability to activate Ypt1-His₇ in the presence of TGN liposomes. Error bars represent 95% CIs for n ≥ 3 reactions. (B) rTRAPPII active site mutants were tested for their ability to activate prenylated-Ypt32/GDI and prenylated-Ypt1/GDI in the presence of TGN liposomes. Error bars represent 95% CIs for n ≥ 2 reactions. (C) rTRAPPII-mediated activation of mantGDP-labeled prenylated-Rab/GDI substrates on TGN liposomes in the absence (mock) or presence of equimolar concentrations of competing unlabeled prenylated-Rab/GDI complexes. Error bars represent 95% CIs for n ≥ 3 reactions. n.s., not significant; *, P < 0.05; **, P < 0.01; ***, P < 0.001.