Figure 3.

Single Cell Analysis of Tumor Infiltrating Treg Cells

(A) Schematic representation of the experimental workflow. Experiments were performed on Treg cells infiltrating CRC, NSCLC, or isolated from peripheral blood of healthy donors (PB); five samples were collected for each tissue.

(B) Percentage of co-expression of signature genes with FOXP3 and IL2RA is depicted.

(C) Expression levels of the signature genes classified by the percentage of co-expression are represented as boxplot.

(D) Expression distribution (violin plots) in Treg cells infiltrating CRC, NSCLC, or PB. Plots representing the ontology classes of receptors, signaling and enzymatic activity, cytokine activity, and transcription factors are shown (Wilcoxon Mann Whitney test p < 0.05). Color gradient indicates the percentage of cells expressing each gene in Treg cells isolated from the three tissues.

(E) Gene-expression analysis of tumor Treg signature genes in different tumor types. Expression values are expressed as log2 (2ˆ-DCt).

See also Figure S3.