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. 2016 Oct 10;5(11):3166–3175. doi: 10.1002/cam4.881

Figure 6.

Figure 6

Effects of ERL on the phosphorylation of c‐Jun and the expression of cleaved caspase‐3 in A549 cells. (A) A549 cells were treated with various concentrations of ERL (0, 5, 15, 25, 35, and 45 μmol/L), and the phosphorylation of c‐Jun and the protein expression of cleaved caspase‐3 were detected by western blot. (B) The intensity of the bands was expressed as optical density (OD) analysis. (C) The effect of NAC on ERL‐induced changes on the ratio of p‐c‐Jun/c‐Jun. (D) The effect of NAC on ERL‐induced changes on the ratio of cleaved caspase‐3/caspase‐3. Data were presented as mean ± SD. *P < 0.05 relative to control group, ns: no significant differences. (E) ERL could increase the level of ROS, which further activates JNK signaling pathway and then lead to A549 cells apoptosis. ERL, erlotinib; c‐Jun N‐terminal kinase; NAC, N‐acetylcysteine; ROS, reactive oxygen species.