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. 2016 Oct 5;5(11):3205–3213. doi: 10.1002/cam4.913

Figure 6.

Figure 6

The expression of AE cleavage was abrogated by caspase‐3 inhibitor. Cells with or without 50 μmol/L caspase‐3 inhibitor Q‐DEVD‐OPh (QDO) were cultured for 16 h with 36 nmol/L homoharringtonine (HHT), 103 nmol/L cytarabine (Ara‐C) or 90 nmol/L aclarubicin (ACR) alone (A, C) and in various combinations (B, D). Western blot analyses of caspase‐3, cleaved caspase‐3 (Δcaspase‐3), PARP, and cleaved PARP (ΔPARP) protein expression in SKNO‐1 cells. Caspase‐3 activation and PARP degradation were significantly inhibited in each group except for the combination of homoharringtonine and aclarubicin, and AML1‐ETO (AE) cleavage was also markedly abrogated following pretreatment with 50 μmol/L caspase‐3 inhibitor QDO for 16 h. *VS QDO group P < 0.05; **VS QDO group P < 0.01.