Figure 5.

The Didemnin B Binding Site

(A) Chemical structure of didemnin B.

(B) Fit of the model of didemnin B (blue) to the EM map density contoured at 5σ.

(C) Didemnin B binds at the interface between the G domain (red) and domain 3 (yellow) of eEF1A. Domain 2 is shown in orange. Relative to the eEF1A crystal structure (PDB: 4C0S; gray), the β15-β16 hairpin packs against didemnin B.

(D) Didemnin B occupies a hydrophobic pocket of eEF1A (orientated as in C), which corresponds to the binding site for kirromycin (green) on EF-Tu.

(E) Hydrogen-bonding interactions between didemnin B (blue) and eEF1A (pink).

See also Figures S5 and S6.