Figure 6.

Interactions between the GTPase and the Ribosome

(A) Comparison of the switch 1 loop (red) of eEF1A (pink) in the elongation complex with the EF-Tu switch 1 loop (teal) in the presence of GMPPCP (PDB: 4V5L) (left). The switch 1 (Sw1) loop interacts with proteins and rRNA from both the large (blue) and small (yellow) subunits of the ribosome (right).

(B) EM map density and model of the interactions between the eEF1A switch 1 loop (red) with rRNA and proteins of the large (blue) and small (yellow) subunit. Density for rRNA is contoured at 9σ; density for eEF1A and uL14 is contoured at 5σ.

(C) Sequence alignment of the switch 1 loop region of selected translational GTPases.