| Complex | Ribosome (∼120 nM) | mRNA substrate (seeFigure S1) | Recombinant proteins (∼600 nM each) | Other |
|---|---|---|---|---|
| 80S⋅aa-tRNA⋅eEF1A | Rabbit (RRL) | Long NC | None | 5 μM didemnin B |
| 80S⋅eRF1⋅eRF3 | Rabbit (RRL) | NC-stop (UGA) | Human WT eRF1 | 1 μM GMPPCP |
| Human WT eRF3 | ||||
| 80S⋅Pelota⋅Hbs1l | Rabbit (RRL) | Trunc. NC OR | Human WT Pelota | 1 μM GMPPCP |
| polyA NC OR | Human WT Hbs1l | |||
| NC-stop (UGA) | ||||
| 80S⋅eRF1 | Rabbit (RRL) | NC-stop (UGA) | Human WT eRF1 | 1 μM GTP |
| Human WT eRF3 | ||||
| 80S⋅eRF1⋅ABCE1 (Brown et al., 2015b) | Rabbit (RRL) | NC-stop | eRF1(AAQ) | None |
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