Fig. 3.

Cytosine methylation levels of single and stacked maize varieties in four regions of the transgenes. The levels of cytosine methylation were measured in CG, CHG and CHH residues. a FMV promoter region; b cry2Ab2 transgene region; c 35S promoter region; d cry1A.105 transgene region. Vertical bars indicate standard errors. Means followed by different letters in the same cytosine type are significantly different according to the Wilcoxon-Mann–Whitney test (p < 0.05). Exact p values for the statistical test of each of the cytosine comparisons are shown on the right upper corner