Table 2.
Bisulfite primers used to amplify four DNA regions after bisulfite conversion
| Primer name | Sequence | GM region | Ta (°C) |
|---|---|---|---|
| BS35S-F | 5′-TTATTAAAAGGATAGTAGAAAAGG-3′ | P-e35S | 51 |
| BS35S-R | 5′-AAAACCTCCCTTAAATCTTATAAT-3′ | ||
| BScry1A.105-F | 5′-ATTATTTGGGGTATTTTTGGTTTTTT-3′ | cry1A.105 | 55 |
| BScry1A.105-R | 5′-AAACCCCACCTCTAACCAAACA-3′ | ||
| BSFMV-F | 5′-GTTTGTGGGGATTAGATAAAAAA-3′ | P-FMV | 50 |
| BSFMV-R | 5′-CACACAAAAAAACATTCAAAAAAA-3′ | ||
| BScry2Ab2-F | 5′-TTTATGTTTTGTTTGTTGTTAGAGTGA-3′ | cry2Ab2 | 51 |
| BScry2Ab2-R | 5′-ACCAAAAATATTCCTAATAAAATAAT-3′ |
Ta annealing temperature, F forward primer, R reverse primer