Table 4.
Description of reference genes and transgenes used for quantification of transcripts and their primer sequences
| Primer name | Gene product | Genbank accession no. | Primer sequence |
|---|---|---|---|
| MEP | Membrane protein PB1A10.07c | GRMZM2G018103_T01 | F-GTACTCGGCAATGCTCTTGA |
| P-AACTTCGGTTGGTGAGAGCGGAAA | |||
| R-CAATCCTGACCCAGACAGATG | |||
| LUG | Leunig | GRMZM2G425377_T01 | F-GGGACATAAGGGAGAAGAACAC |
| P-TTCCCTGTAGCACTGGATGATGCC | |||
| R-TCATGGCTTACTGAGGCAAC | |||
| Cry1A.105 | Cry1A.105 protein | FV532179/FB707509 | F-GACGTGGAGGAACAGAACAA |
| P-TTGTGCCTGAGTGGGAAGCTGAA | |||
| R-CCTCTACCTGGACAGACTCTAA | |||
| Cry2Ab2 | Cry2Ab2 protein | FV532179/AR260587 | F-GCGACTACCTGAAGAACTACAC |
| P-CAACACCTACCAGTCGGCCTTCAA | |||
| R-TGTCGTGAAGCCTCGTATTG |
F forward primer, R reverse primer, P probe