Skip to main content
. 2016 Nov 23;10:270. doi: 10.3389/fncel.2016.00270

Figure 2.

Figure 2

Stretch-induced Interleukin 3 (IL-3) release involves the P2X7R. (A) Stretching isolated neurons with 20 mmHg pressure increased levels of extracellular IL-3 (*p = 0.01, n = 4). The addition of P2X7R antagonists A438079 (10 μM, **p = 0.005 vs. stretched) or Brilliant Blue G (BBG, 1 μM, **p = 0.01 vs. stretched) significantly suppressed the stretch-activated release of IL-3 by 63.2 ± 7.1% and 68.2 ± 10.7%, respectively. Note that the slight inhibition of baseline IL-3 levels by A438079 and BBG were determined to be statistically NS. (B) Incubation of isolated neurons with 50 μM BzATP for 30 min led to a significant increase in extracellular IL-3 concentrations, compared to isotonic control. The BzATP-induced release of IL-3 was attenuated by the removal of calcium from the extracellular solution or by the presence of P2X7 receptor antagonist A438079 (10 μM). (n = 6, *p < 0.05 vs. Control, **p < 0.05 vs. BzATP).