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. 2016 Nov 23;7:1711. doi: 10.3389/fpls.2016.01711

FIGURE 2.

FIGURE 2

Effects of Fe deficiency and high pH on plant Fe status, root Fe uptake machinery and phenylpropanoid pathway components in Arabidopsis thaliana. Plants were pre-grown for 11 days in the presence of 20 μM Fe (III)-EDTA at pH 5.5, and then grown for 14 days in a medium with 0 (-Fe) or 20 μM (+Fe) Fe(III)-EDDHA in nutrient solutions buffered at pH 5.5 (with 5 mM MES-NaOH) or 7.5 (with 5mM HEPES-NaOH). (A) Plants at day 14 after imposing treatments. (B) Leaf chlorophyll concentration in young leaves of plants at day 14 after imposing treatments; data are means ± SE (n = 3) and significant differences among treatments (at p < 0.05) are marked with different letters above the columns. (C) Dry weights and Fe contents in shoots and roots at day 14 after imposing treatments. Data are means ± SE for biomass (n = 5) and for Fe contents (n = 2–5), and significant differences among treatments (at p < 0.05) are marked with different letters above the columns. (D) Abundance of IRT1, FRO2, ABCG37 (PDR9), F6’H1, COMT and CCoAMT transcripts in roots at day 3 after imposing treatments. RNAs were extracted from roots and analyzed by qRT-PCR, using PP2 (At1g13320) as housekeeping gene. The ΔΔCT method was used to determine the relative transcript level. Data are means ± SE (n = 3–5). For each gene, significant differences among treatments (at p < 0.05) are marked with different letters above the columns.