FIGURE 3.
Chromatographic separation of a range of phenolic-type compounds produced in response to Fe deficiency by Arabidopsis thaliana roots. Typical fluorescence (at λexc 365 and λem 460 nm) and absorbance (at 320 nm) chromatograms for root and growth media extracts from plants grown as described in Fourcroy et al. (2014): plants were pre-grown for 29 days in the presence of 45 μM Fe (III)-EDTA at pH 5.5, and then grown for 7 days in a medium with 0 (-Fe) or 45 μM Fe (III)-EDTA (+Fe) (the pH was not readjusted to 5.5, with the final pH being c. 7.0 in all pots). Chromatograms were obtained using Elution program 1. The encircled numbers above each peak correspond to the phenolic compounds listed in Table 1. RU, relative units, AU, absorbance units, and RT, retention time.