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. 2016 Nov 23;7:1711. doi: 10.3389/fpls.2016.01711

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Copyright © 2016 Sisó-Terraza, Luis-Villarroya, Fourcroy, Briat, Abadía, Gaymard, Abadía and Álvarez-Fernández.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of Fe deficiency, high pH and/or time on the relative concentrations of coumarins (scopletin, fraxetin, isofraxidin, fraxinol and total coumarins) in root extracts and nutrient solution (A) and on the allocation of coumarins to the roots and the nutrient solutions of Arabidopsis thaliana(B). Plants were pre-grown as indicated in Figure 2 and grown for 7 or 14 days with 0 (-Fe) or 20 μM Fe (+Fe) in nutrient solution buffered at pH 5.5 (with 5 mM MES-NaOH) or 7.5 (with 5 mM HEPES-NaOH). Data are means of n = 3–5. The absolute values are shown in Figures 5 and 7.