Table 3.
MS/MS data for some of the compounds secreted and accumulated by Arabidopsis thaliana roots in response to Fe deficiency: m/z ratios of the fragment ions and their relative intensity.
| Compound # | Annotation | Parent ion m/z | Ion type | ESI-MSn m/z (Relative intensity, in %) |
|---|---|---|---|---|
| 3 | Coniferylaldehyde hexoside | 339.1 | [M-H]- | MS2 [339]: 295 (6), 275 (8), 250 (6), 249 (3), 188 (3), 177 (100), 162 (3) MS3 [339→177]: 162 (100) |
| 4 | 7,8-dihydroxy-6-methoxycoumarin hexoside (fraxetin hexoside) | 369.1 | [M-H]- | MS2 [369]: 325 (7), 323 (5), 223 (11), 215 (8), 207 (100), 193 (5), 192 (20) MS3 [369→207]: 192 (100) |
| 5 | 7-hydroxy-6,8-dimethoxycoumarin hexoside (isofraxidin hexoside) | 383.1 | [M-H]- | MS2 [383]: 365 (13), 347 (24), 341 (12), 339 (10), 337 (22), 323 (24), 322 (18), 303 (14), 270 (20), 268 (25), 266 (18), 252 (9), 251 (30), 221 (100), 215 (38), 207 (7), 206 (11), 203 (11), 199 (15), 187 (8), 177 (20), 173(8), 156 (11), 131 (17), 129 (30), 125 (6), 114 (24) MS3 [383→221]: 206 (100) |
| 6 | Sinapyl aldehyde hexoside | 369.1 | [M-H]- | MS2 [369]: 351 (33), 325 (11), 289 (10), 254 (5), 253 (6), 246 (11), 245 (8), 239 (9), 237 (11), 217 (6), 207 (100), 192 (18), 159 (11), 128 (10) MS3 [369→207]: 192 (100) |
| 10 | Ferulic acid | 193.1 | [M-H]- | MS2 [193]: 178 (70), 149 (100), 134 (72) |
| 12 | Coniferyl aldehyde | 179.1 | [M+H]+ | MS2 [179]: 161 (86), 147 (100), 133 (17), 119 (10), 105 (8) |
| 13 | Sinapyl aldehyde | 209.1 | [M+H]+ | MS2 [209]: 191 (41), 181 (17), 177 (100), 149 (22), 145 (13), 131 (5), 121 (18) |
Numbers in italics (Compound #) refer to the labels used for each compound in Table 1. All data were taken from the HPLC-ESI-MS/MS(ion trap) analysis. The major ion of the MS2 and MS3 spectra is also indicated in bold.