FIG 2 .

Infection of primary keratinocytes with HPV18 quasivirus and neutralization of infection with Cervarix antiserum. (A) Primary HFKs were infected with HPV18 quasivirus at 100 VGE/cell and incubated for the indicated times. E1^E4 and E6*I spliced early transcripts were detected by qRT-PCR and normalized to human TATA binding protein (TBP) transcripts. (B) Quasivirus was preincubated with the indicated dilutions of rabbit serum obtained pre- and post-vaccination with Cervarix. Primary keratinocytes were infected at 100 VGE/cell, and at 72 h postinfection, viral E1^E4 transcript abundance was determined with qRT-PCR and normalized to human TATA binding protein. Percent neutralization compared to preimmune serum is shown on the right. n = 3; error bars show standard errors of the means.