FIG 6 .

Efficient gene expression is not repressed by the Brd4 binding function of HPV18 E2 upon de novo infection. Quasivirus inocula containing WT HPV18 genomes or genomes mutated in one or both of the key Brd4 binding E2 transactivation domain residues were used to infect HFKs at 100 VGE/cell. (A and B) E1^E4 and E6*1 early spliced transcripts were measured by qRT-PCR 72 h postinfection, corrected to TATA binding protein (TBP) transcripts (A), and normalized to WT (B). n = 3. Error bars show standard errors of the means. (C and D) Total DNA was digested with DpnI, and the abundance of newly replicated HPV18 genomes was detected by qPCR and normalized to β-actin. n = 3. Error bars show standard errors of the means. A paired t test was used for statistical analysis; ns, not significant.