FIG 3 .

I.t. BCG vaccination generates T cells seeding the lung airways. (A) Pie charts illustrating the composition of BALF cell populations as proportions of the total leukocytes from naive mice and mice at 60 days after BCG vaccination. (B) Flow cytometric quantification of total TCRβ⁺ CD4⁺ and CD8⁺ T cells in BALF at the time points indicated after BCG vaccination (gray) and an M. tuberculosis challenge (black). (C, D) Quantification of total BALF TCRβ⁺ Ag85B:H-2I-A^b CD4⁺ and TB10.4:H-2K^b CD8⁺ T cells (C) and TCRβ⁺ Ag85B:H-2I-A^b CD4⁺ and TB10.4:H-2K^b CD8⁺ T cells expressing CXCR3 (D) at designated time points after BCG vaccination. Results are presented as pooled mean data ± the standard error of the mean (B to D) or representative images (A) from two pooled independent experiments (n = 6 to 8 mice per group). The statistical significance of differences between s.c. and i.t. BCG vaccinations is shown. ****, P ≤ 0.0001; ***, P ≤ 0.001; **, P ≤ 0.01; *, P ≤ 0.05. (B to D) Analysis of variance with Tukey’s posttest for significance.