Figure 2.

Immunofluorescence for Aire protein 48 h after anti-Aire siRNA electro-transfection. (A) Thymus electro-transfected with PBS buffer (control) or (B) electro-transfected with anti-Aire siRNA. Nuclei of cells were stained with DAPI (blue) and for AIRE protein in green by using goat anti-Aire IgG primary antibody and fluorescein-conjugated rabbit anti-goat IgG secondary antibody. Comparing to the control thymus, immunofluorescence signal for AIRE protein was strikingly reduced in the nuclei of cells of Aire-knockdown thymus. Magnification 40×, c, cortex; m, medulla.