Figure 1.

Experimental design and intraventricular injection of reprogramming factors. (a) Schematic timeline of the experiment. (b) A brain picture of chronic hypoxic-ischemic brain injury model. Scale bar = 0.5 cm. (c) At postnatal 6 weeks, mice were injected in the right side of lateral ventricle with GFP and the four reprogramming factors, that is, Oct4, Sox2, c-Myc, and Klf4 (treatment group), or GFP only (control group). (d) An image of GFP expression in the lateral ventricle. Scale bar = 200 μm. (e) Absence of dysplasia or tumor development in the lateral ventricle. (f) Absence of dysplasia or tumor development in the hippocampus. Both samples of (e) and (f) were evaluated 8 weeks after the treatment, and brain sections were stained with hematoxylin and eosin and observed using a microscope. Neither dysplasia nor tumors were observed in any group. Scale bars = 200 μm. PAT, passive avoidance task; LV, lateral ventricle; DG, dentate gyrus.