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. 2016 Nov 9;2016:2580837. doi: 10.1155/2016/2580837

Figure 4.

Figure 4

The number of neural progenitor cells in the subventricular zone increased by the in vivo expression of reprogramming factors. (a) The density of BrdU+ cells in the subventricular zone was significantly higher in the treatment group than the control group (t = 3.596, p < 0.01). (b-c) The density of neural progenitor cells was determined through confocal microscopy by calculating the density of cells triple positive for DAPI (blue, nuclei), BrdU (green), and cell type-specific markers such as Nestin and GFAP. The densities of BrdU+Nestin+ cells (b) and BrdU+GFAP+ cells (c) were significantly higher in the treatment group than the control group (t = 3.868, p < 0.01 and t = 2.631, p < 0.05, resp.). (d–g) Confocal microscope images are immunohistochemistry results. (e, g) Cells with triple positive for DAPI, BrdU, and cell type-specific markers are indicated in the yellow box at the right panel. Scale bars = 50 μm. ∗ p < 0.05; ∗∗ p < 0.01.