Figure 7. Lineage tracing of Mfsd2a⁺ hepatocytes after single injection of CCl₄.

(a) Schematic figure showing strategy for tamoxifen induction (Tam), CCl₄ treatment and time points for tissue analysis. (b) Haematoxylin and eosin staining of liver sections collected at day 2 or week 2 after after single CCl₄ injection. C, central vein; P, portal vein. Scale bars, 100 μm. (c,d) Immunostaining for RFP, CK19 and HNF4a on liver sections collected at day 2 (c) and week 2 (d) after single CCl₄ injection. Scale bars, 100 μm. Each image is a representative of four individual samples.