Figure 9. Mfsd2a-derived hepatocytes remain throughout the entire liver lobule after recovery from liver injury.

(a) Schematic figure showing strategy for tamoxifen induction (Tam), CCl₄ treatment and analysis after recovery. (b) Sirius red staining on liver sections collected from CCl₄-treated mice after recovery and control mice (no CCl₄ treatment). Scale bars, 100 μm. (c) Whole-mount bright-field and fluorescence view of liver after recovery from CCl₄ treatment. Scale bars, 1 mm. (d) Immunostaining for RFP on liver sections showing RFP⁺ cells retained throughout entire liver lobules after recovery. Scale bars, 100 μm. (e–g) Immunostaining for RFP, CK19 and HNF4a on liver sections showing most hepatocytes in PP zone (f) and PC zone (g) as RFP⁺; f,g are magnified images of boxed regions in e. Scale bars, 100 μm. Each image is a representative of four individual samples.