Figure 1. Usp21^fl/flFoxp3^Cre mice develop spontaneous lymphoproliferative disease.

(a) CD4⁺CD25⁻YFP⁻ effector T (Teff) cells and CD4⁺CD25^hiYFP⁺ Treg cells were sorted from Foxp3^Cre (WT) and Usp21^fl/flFoxp3^Cre (KO) mice. mRNA expression of Usp21 in each population was assessed by qRT–PCR. (n=3 for each group). (b) Image of spleens and peripheral lymph nodes (pLNs) from 8-month-old WT and KO mice. (c) Quantitative analysis of the weight of spleens isolated from WT (n=5) and KO (n=5) mice. (d) Representative figure shown the expression of CD62L and CD44 in splenic CD4⁺YFP⁻ and CD8⁺ T cells from WT (n=7) and KO (n=7) littermates. (e) Percentage of splenic CD62L^loCD44^hi effector memory T cells as in d. (f) Representative figure shown the expression of IFN-γ, IL-17, IL-2 and IL-4 by splenic CD4⁺YFP⁻ effector T (Teff) cells from WT (n=5) and KO (n=5) mice. (g) Percentage of IFN-γ⁺, IL-17⁺, IL-2⁺ and IL-4⁺ splenic T cells as in f. Small horizontal lines indicate the mean (±s.d.). All data represent means±s.d. **P≤0.01, as determined by Student's t-test.