Table 3.

Comparison of spatial fluctuations of PTM sites in 3-D structure before and after modifications. PTMs with one or more modified residue in NMR-based 3-D structure are included. The p-value for the significance of the fluctuation difference is based on two-tailed, two-sample student t-test.

			Modified in structure		Pre-modified in structure
PTM	UniProt keyword	Amino acid	# of sites	Mean fluctuation (Å)	# of sites	Mean fluctuation (Å)	p-value
Phosphorylation	Phosphoserine	S	9	0.98	576	1.90	8.2E-4
Phosphorylation	Phosphothreonine	T	15	1.07	116	1.69	0.022
Phosphorylation	Phosphotyrosine	Y	3	0.93	161	1.10	0.447
Methylation	N6-methyllysine	K	10	0.94	18	1.84	0.006
Dimethylation	N6,N6-dimethyllysine	K	5	0.84	8	2.90	2.6E-04
Trimethylation	N6,N6,N6-trimethyllysine	K	12	0.71	20	1.18	0.073
Hydroxylation	4-hydroxyproline	P	65	0.74	3	0.71	0.917
Acetylation	N6-acetyllysine	K	7	0.95	388	1.02	0.442
Sulfation	Sulfotyrosine	Y	4	0.93	2	1.19	0.001
Carboxylation	4-carboxyglutamate	E	49	1.76	13	3.52	0.004