Table 5. The effects of pre-weaning (gestation and lactation) or post-weaning diets (canned or kibbled) on the enzymes involved in key vitamin biosynthesis and metabolism related pathways the Ubiquinone and other terpenoid-quinone biosynthesis [PATH:ko00130], Thiamine metabolism [PATH:ko00730], Sulfur relay system [PATH:ko04122], Riboflavin metabolism [PATH:ko00740], Vitamin B6 metabolism [PATH:ko00750], Nicotinate and nicotinamide metabolism [PATH:ko00760], Pantothenate and CoA biosynthesis [PATH:ko00770], Biotin metabolism [PATH:ko00780], and Folate biosynthesis [PATH:ko00790] KEGG orthology pathways (proportion of total sequences) present in faecal samples of the domestic kitten (Felis catus; n = 5 per treatment).
K-K | K-C | C-K | C-C | Pre-weaning diet1 | Post-weaning diet2 | Pre x Post-weaning | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
KEGG ID | Mean | SEM | Mean | SEM | Mean | SEM | Mean | SEM | P-value | FDR | P-value | FDR | P-value | FDR | |
Ubiquinone and other terpenoid-quinone biosynthesis [PATH:ko00130] | |||||||||||||||
Menb; [EC:4.1.3.36] | K01661 | 0.003 | 0.001 | 0.136 | 0.033 | 0.055 | 0.029 | 0.168 | 0.036 | 0.143 | 0.321 | 0.003 | 0.008 | 0.726 | 0.838 |
Menh; [EC:4.2.99.20] | K08680 | 0.000 | 0.000 | 0.005 | 0.002 | 0.000 | 0.000 | 0.000 | 0.000 | 0.008 | 0.029 | 0.001 | 0.005 | 0.025 | 0.075 |
Ubib, aarf | K03688 | 0.086 | 0.035 | 0.008 | 0.008 | 0.046 | 0.046 | 0.033 | 0.027 | 0.820 | 0.914 | 0.214 | 0.269 | 0.267 | 0.466 |
Ubif; [EC:1.14.13.-] | K03184 | 0.000 | 0.000 | 0.012 | 0.005 | 0.000 | 0.000 | 0.000 | 0.000 | 0.001 | 0.007 | 0.001 | 0.005 | 0.001 | 0.005 |
Ubih; [EC:1.14.13.-] | K03185 | 0.000 | 0.000 | 0.009 | 0.003 | 0.001 | 0.000 | 0.000 | 0.000 | 0.001 | 0.007 | 0.001 | 0.005 | 0.001 | 0.005 |
Ubix; [EC:4.1.1.-] | K03186 | 0.000 | 0.000 | 0.007 | 0.004 | 0.000 | 0.000 | 0.000 | 0.000 | 0.009 | 0.032 | 0.048 | 0.076 | 0.041 | 0.111 |
Mend; [EC:2.2.1.9] | K02551 | 0.000 | 0.000 | 0.075 | 0.029 | 0.000 | 0.000 | 0.057 | 0.036 | 0.639 | 0.810 | 0.017 | 0.033 | 0.670 | 0.802 |
Menf; [EC:5.4.4.2] | K02552 | 0.000 | 0.000 | 0.009 | 0.004 | 0.000 | 0.000 | 0.001 | 0.001 | 0.003 | 0.013 | 0.001 | 0.005 | 0.007 | 0.024 |
Mena; [EC:2.5.1.74/EC:2.5.1.-] | K02548 | 0.000 | 0.000 | 0.000 | 0.000 | 0.033 | 0.012 | 0.000 | 0.000 | 0.003 | 0.013 | 0.002 | 0.006 | 0.003 | 0.012 |
Ubia; [EC:2.5.1.-] | K03179 | 0.000 | 0.000 | 0.008 | 0.004 | 0.001 | 0.001 | 0.000 | 0.000 | 0.027 | 0.083 | 0.068 | 0.100 | 0.012 | 0.039 |
Ubic; [EC:4.1.3.40] | K03181 | 0.000 | 0.000 | 0.007 | 0.002 | 0.000 | 0.000 | 0.000 | 0.000 | 0.001 | 0.007 | 0.001 | 0.005 | 0.001 | 0.005 |
Ubie; [EC:2.1.1.163/EC:2.1.1.201] | K03183 | 0.000 | 0.000 | 0.023 | 0.010 | 0.004 | 0.002 | 0.016 | 0.016 | 0.891 | 0.955 | 0.097 | 0.134 | 0.591 | 0.753 |
Entc; [EC:5.4.4.2] | K02361 | 0.025 | 0.015 | 0.011 | 0.007 | 0.000 | 0.000 | 0.000 | 0.000 | 0.019 | 0.061 | 0.322 | 0.378 | 0.342 | 0.538 |
Ubig; [EC:2.1.1.222/EC:2.1.1.64] | K00568 | 0.000 | 0.000 | 0.005 | 0.002 | 0.000 | 0.000 | 0.000 | 0.000 | 0.001 | 0.007 | 0.008 | 0.018 | 0.003 | 0.012 |
Ubid; [EC:4.1.1.-] | K03182 | 0.001 | 0.001 | 0.024 | 0.013 | 0.000 | 0.000 | 0.000 | 0.000 | 0.007 | 0.025 | 0.034 | 0.057 | 0.029 | 0.084 |
Thiamine metabolism [PATH:ko00730] | |||||||||||||||
Thil; [EC:2.7.4.16] | K01516 | 0.090 | 0.037 | 0.144 | 0.051 | 0.093 | 0.036 | 0.191 | 0.025 | 0.529 | 0.730 | 0.063 | 0.094 | 0.569 | 0.742 |
Thi80; [ec:2.7.6.2] | K00949 | 0.036 | 0.015 | 0.000 | 0.000 | 0.010 | 0.010 | 0.007 | 0.007 | 0.317 | 0.532 | 0.043 | 0.069 | 0.105 | 0.239 |
Tena; [EC:3.5.99.2] | K03707 | 0.001 | 0.000 | 0.003 | 0.003 | 0.034 | 0.015 | 0.009 | 0.009 | 0.052 | 0.145 | 0.241 | 0.296 | 0.112 | 0.250 |
Thig | K03149 | 0.135 | 0.076 | 0.327 | 0.051 | 0.197 | 0.056 | 0.264 | 0.043 | 0.994 | 0.996 | 0.049 | 0.077 | 0.303 | 0.501 |
Thih | K03150 | 0.105 | 0.096 | 0.703 | 0.106 | 0.117 | 0.080 | 0.530 | 0.056 | 0.367 | 0.573 | 0.001 | 0.005 | 0.307 | 0.503 |
Thik; [EC:2.7.1.89] | K07251 | 0.000 | 0.000 | 0.004 | 0.001 | 0.000 | 0.000 | 0.000 | 0.000 | 0.001 | 0.007 | 0.001 | 0.005 | 0.001 | 0.005 |
Sufs; [EC:2.8.1.7/EC:4.4.1.16] | K11717 | 0.142 | 0.058 | 0.128 | 0.059 | 0.029 | 0.029 | 0.164 | 0.068 | 0.539 | 0.737 | 0.297 | 0.354 | 0.181 | 0.360 |
Sulfur relay system [PATH:ko04122] | |||||||||||||||
Thif; [EC:2.7.7.73] | K03148 | 0.012 | 0.012 | 0.004 | 0.004 | 0.031 | 0.017 | 0.014 | 0.014 | 0.269 | 0.493 | 0.354 | 0.411 | 0.652 | 0.788 |
Thii | K03151 | 0.101 | 0.041 | 0.007 | 0.007 | 0.041 | 0.029 | 0.022 | 0.022 | 0.374 | 0.579 | 0.049 | 0.077 | 0.229 | 0.420 |
This | K03154 | 0.003 | 0.003 | 0.000 | 0.000 | 0.009 | 0.004 | 0.004 | 0.004 | 0.155 | 0.341 | 0.216 | 0.271 | 0.740 | 0.848 |
Riboflavin metabolism [PATH:ko00740] | |||||||||||||||
Fre, ubib; [EC:1.16.1.3/EC:1.5.1.41] | K05368 | 0.000 | 0.000 | 0.009 | 0.003 | 0.000 | 0.000 | 0.000 | 0.000 | 0.001 | 0.007 | 0.001 | 0.005 | 0.001 | 0.005 |
Ribb, RIB3; [EC:4.1.99.12] | K02858 | 0.085 | 0.030 | 0.265 | 0.024 | 0.099 | 0.069 | 0.307 | 0.041 | 0.559 | 0.751 | 0.002 | 0.006 | 0.745 | 0.851 |
Ribd; [EC:3.5.4.26/EC:1.1.1.193] | K11752 | 0.070 | 0.029 | 0.000 | 0.000 | 0.025 | 0.015 | 0.020 | 0.020 | 0.501 | 0.705 | 0.058 | 0.088 | 0.100 | 0.232 |
Ribe, RIB5; [EC:2.5.1.9] | K00793 | 0.042 | 0.017 | 0.065 | 0.029 | 0.000 | 0.000 | 0.073 | 0.020 | 0.427 | 0.631 | 0.034 | 0.057 | 0.194 | 0.377 |
Ribh, RIB4; [EC:2.5.1.78] | K00794 | 0.050 | 0.010 | 0.115 | 0.011 | 0.041 | 0.023 | 0.124 | 0.019 | 0.977 | 0.986 | 0.001 | 0.005 | 0.607 | 0.761 |
Ssue; [EC:1.5.1.38] | K00299 | 0.000 | 0.000 | 0.004 | 0.001 | 0.000 | 0.000 | 0.000 | 0.000 | 0.001 | 0.007 | 0.002 | 0.006 | 0.002 | 0.008 |
Apha; [EC:3.1.3.2] | K03788 | 0.000 | 0.000 | 0.008 | 0.003 | 0.000 | 0.000 | 0.000 | 0.000 | 0.006 | 0.022 | 0.002 | 0.006 | 0.002 | 0.008 |
Epd; [EC:1.2.1.72] | K03472 | 0.000 | 0.000 | 0.013 | 0.005 | 0.000 | 0.000 | 0.000 | 0.000 | 0.004 | 0.017 | 0.001 | 0.005 | 0.002 | 0.008 |
Pdxs, pdx1; [EC:4.-.-.-] | K06215 | 0.210 | 0.054 | 0.207 | 0.029 | 0.252 | 0.060 | 0.157 | 0.025 | 0.922 | 0.968 | 0.261 | 0.317 | 0.297 | 0.496 |
Pdxj; [EC:2.6.99.2] | K03474 | 0.000 | 0.000 | 0.055 | 0.010 | 0.001 | 0.001 | 0.066 | 0.018 | 0.569 | 0.759 | 0.001 | 0.005 | 0.601 | 0.758 |
Nicotinate and nicotinamide metabolism [PATH:ko00760] | |||||||||||||||
Iunh; [EC:3.2.2.1] | K01239 | 0.000 | 0.000 | 0.002 | 0.002 | 0.118 | 0.052 | 0.017 | 0.017 | 0.007 | 0.025 | 0.107 | 0.145 | 0.087 | 0.208 |
Nada; [EC:2.5.1.72] | K03517 | 0.065 | 0.027 | 0.077 | 0.052 | 0.000 | 0.000 | 0.117 | 0.059 | 0.773 | 0.885 | 0.145 | 0.190 | 0.224 | 0.417 |
Nadc, QPRT; [EC:2.4.2.19] | K00767 | 0.066 | 0.027 | 0.175 | 0.027 | 0.000 | 0.000 | 0.190 | 0.026 | 0.283 | 0.509 | 0.001 | 0.005 | 0.087 | 0.208 |
Nadd; [EC:2.7.7.18] | K00969 | 0.074 | 0.036 | 0.000 | 0.000 | 0.030 | 0.018 | 0.009 | 0.009 | 0.438 | 0.643 | 0.015 | 0.030 | 0.224 | 0.417 |
Pncb, NAPRT1; [EC:2.4.2.11] | K00763 | 0.165 | 0.067 | 0.000 | 0.000 | 0.150 | 0.065 | 0.027 | 0.027 | 0.901 | 0.960 | 0.013 | 0.026 | 0.640 | 0.788 |
Pnta; [EC:1.6.1.2] | K00324 | 0.023 | 0.013 | 0.016 | 0.003 | 0.060 | 0.021 | 0.003 | 0.002 | 0.342 | 0.553 | 0.016 | 0.031 | 0.052 | 0.136 |
Pntb; [EC:1.6.1.2] | K00325 | 0.037 | 0.022 | 0.013 | 0.003 | 0.089 | 0.017 | 0.007 | 0.002 | 0.137 | 0.311 | 0.004 | 0.010 | 0.054 | 0.140 |
Ppnk, NADK;[EC:2.7.1.23] | K00858 | 0.060 | 0.025 | 0.014 | 0.014 | 0.064 | 0.039 | 0.012 | 0.012 | 0.982 | 0.988 | 0.071 | 0.103 | 0.928 | 0.968 |
Puna; [EC:2.4.2.1] | K03783 | 0.061 | 0.025 | 0.083 | 0.038 | 0.000 | 0.000 | 0.126 | 0.033 | 0.723 | 0.858 | 0.019 | 0.036 | 0.070 | 0.174 |
Stha, udha; [EC:1.6.1.1] | K00322 | 0.000 | 0.000 | 0.014 | 0.005 | 0.000 | 0.000 | 0.000 | 0.000 | 0.002 | 0.010 | 0.002 | 0.006 | 0.001 | 0.005 |
Yjjg; [EC:3.1.3.5] | K08723 | 0.000 | 0.000 | 0.007 | 0.003 | 0.000 | 0.000 | 0.000 | 0.000 | 0.001 | 0.007 | 0.001 | 0.005 | 0.001 | 0.005 |
5’-nucleotidase; [EC:3.1.3.5] | K01081 | 0.001 | 0.000 | 0.007 | 0.007 | 0.008 | 0.005 | 0.000 | 0.000 | 0.811 | 0.908 | 0.767 | 0.807 | 0.220 | 0.411 |
Nade; [EC:6.3.1.5] | K01916 | 0.152 | 0.014 | 0.000 | 0.000 | 0.068 | 0.037 | 0.000 | 0.000 | 0.054 | 0.150 | 0.001 | 0.005 | 0.048 | 0.128 |
Pnca; [EC:3.5.1.19 3.5.1.-] | K08281 | 0.011 | 0.007 | 0.000 | 0.000 | 0.062 | 0.029 | 0.000 | 0.000 | 0.086 | 0.222 | 0.001 | 0.005 | 0.075 | 0.184 |
Usha; [EC:3.1.3.5 3.6.1.45] | K11751 | 0.000 | 0.000 | 0.013 | 0.007 | 0.000 | 0.000 | 0.000 | 0.000 | 0.017 | 0.055 | 0.039 | 0.063 | 0.013 | 0.042 |
Pantothenate and CoA biosynthesis [PATH:ko00770] | |||||||||||||||
Acps; [EC:2.7.8.7] | K00997 | 0.027 | 0.011 | 0.003 | 0.003 | 0.024 | 0.015 | 0.008 | 0.008 | 0.863 | 0.939 | 0.063 | 0.094 | 0.662 | 0.795 |
Pand; [EC:4.1.1.11] | K01579 | 0.000 | 0.000 | 0.062 | 0.006 | 0.010 | 0.008 | 0.057 | 0.012 | 0.704 | 0.852 | 0.001 | 0.005 | 0.370 | 0.569 |
Pane, apba; [EC:1.1.1.169] | K00077 | 0.080 | 0.023 | 0.016 | 0.005 | 0.026 | 0.019 | 0.016 | 0.011 | 0.100 | 0.245 | 0.043 | 0.069 | 0.125 | 0.275 |
Coaa; [EC:2.7.1.33] | K00867 | 0.045 | 0.018 | 0.014 | 0.006 | 0.057 | 0.030 | 0.000 | 0.000 | 0.958 | 0.982 | 0.031 | 0.054 | 0.464 | 0.654 |
Coaw; [EC:2.7.1.33] | K09680 | 0.000 | 0.000 | 0.009 | 0.004 | 0.000 | 0.000 | 0.002 | 0.002 | 0.115 | 0.270 | 0.019 | 0.036 | 0.088 | 0.209 |
Panb; [EC:2.1.2.11] | K00606 | 0.000 | 0.000 | 0.080 | 0.020 | 0.004 | 0.004 | 0.085 | 0.025 | 0.751 | 0.876 | 0.001 | 0.005 | 0.989 | 0.995 |
Panc; [EC:6.3.2.1] | K01918 | 0.000 | 0.000 | 0.114 | 0.005 | 0.000 | 0.000 | 0.068 | 0.028 | 0.158 | 0.344 | 0.001 | 0.005 | 0.160 | 0.326 |
Acph; [EC:3.1.4.14] | K08682 | 0.000 | 0.000 | 0.006 | 0.002 | 0.000 | 0.000 | 0.000 | 0.000 | 0.002 | 0.010 | 0.004 | 0.010 | 0.003 | 0.012 |
Biotin metabolism [PATH:ko00780] | |||||||||||||||
Biod; [EC:6.3.3.3] | K01935 | 0.000 | 0.000 | 0.025 | 0.011 | 0.000 | 0.000 | 0.008 | 0.008 | 0.332 | 0.544 | 0.031 | 0.054 | 0.346 | 0.542 |
Bira; [EC:6.3.4.15] | K03524 | 0.059 | 0.024 | 0.000 | 0.000 | 0.018 | 0.018 | 0.014 | 0.014 | 0.323 | 0.537 | 0.051 | 0.079 | 0.118 | 0.262 |
Biof; [EC:2.3.1.47] | K00652 | 0.002 | 0.001 | 0.112 | 0.032 | 0.041 | 0.033 | 0.093 | 0.038 | 0.689 | 0.842 | 0.019 | 0.036 | 0.307 | 0.503 |
Folate biosynthesis [PATH:ko00790] | |||||||||||||||
Folc; [EC:6.3.2.12 6.3.2.17] | K11754 | 0.147 | 0.060 | 0.000 | 0.000 | 0.060 | 0.037 | 0.023 | 0.023 | 0.418 | 0.622 | 0.023 | 0.042 | 0.174 | 0.348 |
Folp; [EC:2.5.1.15] | K00796 | 0.054 | 0.022 | 0.000 | 0.000 | 0.020 | 0.017 | 0.010 | 0.010 | 0.410 | 0.613 | 0.045 | 0.072 | 0.144 | 0.302 |
Paba; [EC:2.6.1.85] | K01664 | 0.029 | 0.017 | 0.008 | 0.004 | 0.043 | 0.016 | 0.009 | 0.007 | 0.526 | 0.727 | 0.037 | 0.061 | 0.592 | 0.753 |
Pabc; [EC:4.1.3.38] | K02619 | 0.000 | 0.000 | 0.003 | 0.003 | 0.034 | 0.017 | 0.009 | 0.009 | 0.038 | 0.111 | 0.275 | 0.331 | 0.198 | 0.382 |
Suld; [EC:4.1.2.25/EC:2.7.6.3] | K13940 | 0.038 | 0.024 | 0.000 | 0.000 | 0.070 | 0.024 | 0.008 | 0.008 | 0.287 | 0.511 | 0.020 | 0.037 | 0.474 | 0.660 |
Results are presented as mean and standard error of the mean (SEM). P value indicates ANOVA significance of rank transformed data and False Discovery Rate (FDR) indicates multiple testing adjusted P value.
K = Kibbled.
C = Canned.
Diet K-Diet K (K-K) n = 3 females, n = 2 males.
Diet C-Diet K (C-K) n = 3 females, n = 2 males.
Diet K-Diet C (K-C) n = 3 females, n = 2 males.
Diet C-Diet C (C-C) n = 4 females, n = 1 male.