Table 1. Results of pathology analysis.
| Mouse | Tumour Score |
||||
|---|---|---|---|---|---|
| Liver | Uterus | Ovary | Kidney | Spleen | |
| sh492 A | 0 | 0 | 4 | 0 | 0 |
| sh492 B | 1 | 0 | 2 | 0 | 0 |
| sh492 C | 0 | 0 | 0 | 0 | 0 |
| sh492 D | 0 | — | — | 0 | 0 |
| TWIST1 A | 0 | 0 | 4 | 0 | 0 |
| TWIST1 B | 0 | — | 4 | 0 | 1 |
| TWIST1 C | 1 | 0 | 4 | 0 | 0 |
| TWIST1 D | 1 | 0 | 4 | 0 | 0 |
TWIST1 overexpressing cells gave rise to large ovarian tumours in 4/4 mice, whereas sh492 expressing cells gave rise to tumours in 2/4 mice, with only one matching the severity seen in TWIST1 tumours (1/4 sh492 scored 4 vs 4/4 TWIST1 scored 4). 3/4 mice receiving TWIST1-expressing cells developed a metastatic lesion in their liver or spleen, compared to 1/4 sh492 mice. A, B, C, and D refer to individual mice. “0” reflects a tumour score of 0, while “—” denotes no sample collected.