Table 1. Sequences of RNA and DNA Oligonucleotides.
Name | Sense Strand/Sense Primer (5′-3′) | Sense Strand/Sense Primer (5′-3′) |
---|---|---|
miRNA and siRNA duplexes | ||
miR-706 | AGAGAAACCCUGUCUCAAAAAA | UGAGACAGGGUUUCUCUUUUU |
miR-NC | UUGUACUACACAAAAGUACUG | GUACUUUUGUGUAGUACAAUU |
anti-miR-706 | UUUUUUGAGACAGGGUUUCUCU | |
anti-NC | CAGUACUUUUGUGUAGUACAA | |
siTaok1 (human) | GCAGUAUGGGAGUCCGCAATT | UUGCGGACUCCCAUACUGCTT |
siPrkca (human) | AGGAACCACAAGCAGUATT | UACUGCUUGUGGUUCCUUATT |
miR-706 Agomir | AGAGAAACCCUGUCUCAAAAAA | UUUUGAGACAGGGUUUCUCUUU |
miR-706 Agomir-NC | UUGUACUACACAAAAGUACUG | GUACUUUUGUGUAGUACAAUU |
miR-706 Antagomir | UUUUUUGAGACAGGGUUUCUCU | |
miR-706 Antagomir-NC | CAGUACUUUUGUGUAGUACAA | |
Primers for qPCR or RT-PCR | ||
HNF-4 (mouse) | CTGAAGGTGCCAACCTCAAT | CACATTGTCGGCTAAACCTG |
α-SMA (mouse) | TTCCTTCGTGACTACTGCTGAG | CAATGAAAGATGGCTGGAAGAG |
α-SMA (human) | ATCAAGGAGAAACTGTGTTATGTAG | GATGAAGGATGGCTGGAACAGGGTC |
GAPDH (mouse) | AACTTTGGCATTGTGGAAGG | CACATTGGGGGTAGGAACAC |
GAPDH (human) | GAGTCAACGGATTTGGTCGT | GACAAGCTTCCCGTTCTCAG |
Bad (mouse) | AAGTCCGATCCCGGAATCC | GCTCACTCGGCTCAAACTCT |
Bad (human) | GAGCCCGGGGTGCTGGAGGGA | GGCGGCACAGACGCGGGCTTT |
Bak1 (mouse) | GTCAGGCAGGTGACAAGTGAG | TTAGTCCAGGCAGTCATGTGG |
Bak1 (human) | GCTCCCAACCCATTCACTAC | TCCCTACTCCTTTTCCCTGA |
Bax (human) | ATGGACGGGTCCGGGGAGCA | CCCAGTTGAAGTTGCCGTCA |
Bax (mouse) | TGAAGACAGGGGCCTTTTTG | AATTCGCCGGAGACACTCG |
Desmin (mouse) | AGGAGAGCAGGATCAACCTT | CCTGGCTTACAGCACTTCAT |
Bbc3 (human) | GGACGACCTCAACGCACAGT | AATTGGGCTCCATCTCGGGG |
Bbc3 (mouse) | AGCAGCACTTAGAGTCGCC | CCTGGGTAAGGGGAGGAGT |
Col1a1(mouse) | GCTCCTCTTAGGGGCCACT | CCACGTCTCACCATTGGGG |
Col1a1(human) | AGCCAGCAGATCGAGAACAT | CAGTTCTTCTGGGCCACACT |
U6 | CTCGCTTCGGCAGCACA | AACGCTTCACGAATTTGCGT |
18S | AAACGGCTACCACATCCAAG | CCTCCAATGGATCCTCGTTA |
mmu-miR-706-LP | CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGTTTTTTGA | |
mmu-miR-706-PF | ACACTCCAGCTGGGACAGAAACCCTGTCTC | |
mmu-miR-706-PR | TGGTGTCGTGGAGTCG | |
Primers for cloning (Restriction enzyme sites were underlined) | ||
miR-706 | AGAGAAACCCUGUCUCAAAAAA | |
Prkca-3′UTR | CCACCUGACAGUCAUUUUCUCAC | |
Taok1-3′UTR | GUUAUAUGGAAACAGGUUUCUCU |