Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Nov 23;6:37223. doi: 10.1038/srep37223

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

Control and Ad-Raptor^KO mice were either kept at thermoneutrality (warm; 30 °C) or exposed to cold (10 °C) for 2 weeks. (A) Western blots performed on BAT following warm or acute cold exposure. All gels have been run under the same experimental conditions. (B) Representative pictures of BAT and (C) H&E staining of representative BAT collected after warm or chronic cold exposure. (D) Total BAT mass (n = 13–17; mean +/− SEM; Two-way ANOVA; ***P < 0.001 vs. Warm; ^###P < 0.001 vs. Control). (E) Total DNA content (n = 7–9; mean +/− SEM; Two-way ANOVA; ***P < 0.001 vs. Warm; ^###P < 0.001 vs. Control). (F) mtDNA content (n = 7–9; mean +/− SEM; Two-way ANOVA; ***P < 0.001 vs. Warm; ^###P < 0.001 vs. Control). (G) Gene expression of Pgc1a (n = 4; mean +/− SEM; Two-way ANOVA; ***P < 0.001 vs. Warm; ^###P < 0.001 vs. Control). (H) Western blots performed on BAT collected from control and Ad-Raptor^KO mice following chronic cold exposure. All gels were run under the same experimental conditions. (I) Oxygen consumption at thermoneutrality (warm) and during the transition to cold. (n = 6–8; mean +/− SEM; t-test; *P < 0.05; ***P < 0.001). White section: thermoneutrality, when the lights were on; Grey section: thermoneutrality, when the lights were off; Light blue section: cold, when the lights were on; Dark blue section: cold, when the lights were off. (J) Mean SUV time-activity curves of ¹¹C-acetate during (left) acute and (right) chronic cold exposure (n = 4–5; mean +/− SEM). (K) Total BAT oxidative activity index corrected for tissue weight (n = 6–8; mean +/−SEM; Two-way ANOVA; ***P < 0.001 vs. Warm; ^#P < 0.05 vs. Control; ^###P < 0.001 vs. Control). (L) Total BAT blood flow index corrected for tissue weight (n = 4–5; mean +/− SEM; Two-way ANOVA; ***P < 0.001 vs. Warm; ^#P < 0.05 vs. Control; ^###P < 0.001 vs. Control). (M) Plasma CK activity (n = 10–13; mean +/− SEM; Two-way ANOVA; ***P < 0.001 vs. Warm; ^###P < 0.001 vs. Control).