Figure 5. mTORC1 loss severely impairs lipid metabolism in BAT following chronic cold exposure.

Control and Ad-Raptor^KO mice were cold exposed for 14 days and fasted during 6 hours before BAT collection. (A) Metabolites entering in the synthesis of glycerolipids from glucose measured by metabolomics. Metabolites in bold are statistically different between control and Ad-Raptor^KO mice (n = 3; mean +/− SEM; t-test; *P < 0.05). (B) Diacylglycerol (DG) species measured by metabolomics. Metabolites in bold are statistically different between control and Ad-Raptor^KO mice (n = 3; mean +/− SEM; t-test; *P < 0.05). (C) Expression of genes entering in the synthesis or the breakdown of glycerolipids extracted from the microarray dataset. Genes in bold are statistically different between control and Ad-Raptor^KO mice (n = 4; mean +/− SEM; SAM t-test; *FDR < 0.05). (D) Lipogenic metabolites produced from the TCA cycle measured by metabolomics (n = 3; mean +/− SEM; t-test). (E) Lipogenic gene expression extracted from the microarray dataset. Genes in bold are statistically different between control and Ad-Raptor^KO mice (n = 4; mean +/− SEM; SAM t-test; *FDR < 0.05). (F) Schematic summary of transcriptomics and metabolomics data.