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. 2016 Nov 23;6:37267. doi: 10.1038/srep37267

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Copyright © 2016, The Author(s)

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(A) IRAK-4 full length was cleaved with thrombin and TEV proteases and analysed by XX% SDS-PAGE (B) Cleaved IRAK-4 was fractionated by gel filtration on 16/60 Superdex™ 75 pg (C) SDS-PAGE analysis of gel filtration peaks 1–3. (D) LC/MS spectrum showing deconvoluted masses of IRAK-4 DD purified in peak 3. The mass of 12782 Da corresponded to IRAK-4 death domain residues (−7) to 107 (see Methods). The mass of 12862 Da represents IRAK-4 death domain with a phosphorylated residue (+80 Da) (C).