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. 2016 Nov 23;6:37267. doi: 10.1038/srep37267

Figure 6. Regulatory function of the IRAK-4 N-terminal loop.

Figure 6

(A) HEK293/TLR4-MD2-CD14 cells were transiently transfected with 10 ng or 20 ng of Myc-tagged IRAK4 constructs. Cells were harvested 24 hours post-transfection. NF-κB activation was quantified by dual luciferase assay. One representative of three independent experiments is shown. Statistical Analysis—p values were determined using multiple t test and designated with p < 0.01 (**) and p < 0.05 (*) One experiment of three biological replicates is shown. (B) Expression of all IRAK4 mutants is comparable with wild type IRAK4. HEK293/TLR4-MD2-CD14 cells were transfected with Myc-tagged IRAK4 constructs and analyzed by immunoblot (IB). One experiment of three biological replicates is shown. (C) Lumier assays: MyD88 and IRAK were tagged with renilla (Ren) or protein A (ProA) at the N- or C-terminal, as indicated. KD = kinase dead. One representative out of three identical experiments shown. **p < 0.01 measured by Student’s t-test.