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. 2016 Nov 10;97(11):3024–3029. doi: 10.1099/jgv.0.000617

Fig. 2.

Fig. 2.

Effect of wMelPop on CFAV and PCLV infection in Aag2 cells. (a) Detection of CFAV, Wolbachia or PCLV in Aag2, Aag2wMelPop and two different cultures of Aag2wMelPop cells treated with tetracycline (Aag2wMelPop-tet sets 1 and 2) cells by RT-PCR. Actin was used as loading control. (b) Detection of CFAV or PCLV in C6/36 cells incubated with supernatant of Aag2, Aag2wMelPop or Aag2wMelPop treated with tetracycline (two different cultures, Aag2wMelPop-tet sets 1 and 2) by RT-PCR. Actin was used as a loading control. (c) Quantification of CFAV RNA in Aag2wMelPop (Wol) or Aag2wMelPop treated with tetracycline (Tet) cells after incubation with Aag2 supernatant containing CFAV by SYBR Green. S7 was used as internal control. Relative RNA expression is represented as (CFAV/S7). Error bars show sem from three independent experiments. (d) Quantification of PCLV RNA in Aag2wMelPop (Wol) or Aag2wMelPop treated with tetracycline (Tet) cells, either after incubation with Aag2 supernatant harbouring PCLV or untreated by SYBR Green. S7 was used as an internal control. Relative RNA expression is represented as (PCLV/S7) and mock-infected tetracycline cells were set to 1. Error bars show sem from three independent experiments. *P≤0.05.