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. 2016 Nov 22;7:23. doi: 10.1186/s13100-016-0079-3

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — The MAPK p38δ phosphorylates ORF1p on S/T-P motifs required for L1 retrotransposition. a ORF1p-WT or S/T-P mutants (200 μM), purified from E. coli, were incubated with 85 nM activated p38δ-WT (top) or the constitutively active p38δ mutant F324S (bottom) in the presence of [γ-³²P]-ATP; bands on autoradiogram show ³²P incorporation into ORF1p. ORF1p mutants are S18A/S27A/T203G/T213G (AAGG), S18A/S27A (AA), T203G/T213G (GG), S27A/T203G/T213G (SAGG), S18A/T203G/T213G (ASGG), S18A/S27A/T213G (AATG) and S18A/S27A/T203G (AAGT). b ORF1p-WT was incubated with activated p38δ-WT, p38δ-F324S, an inactive p38δ mutant D176A, or no kinase in reactions as described in (a). c A Coomassie-stained gel shows each ORF1p construct (approximately 100 ng) purified from E. coli.