Table 3.
PCR Primers: Proposed PCR primers to be used to amplify the 7 MLST loci
| Gene | Forward | Reverse |
|---|---|---|
| MHO_4840a
GENE-20 |
AATGAACCTATTTATTTTTTGGGTG | AATTTTGAATAAACTGGTATTTCTTTG |
| MHO_0720a
GENE-58 |
CTGCTGCAGCACTTATTGC | GAACGTGATAAAGGAACTACTCA and CGCGATAAAGGAACTACTCA b |
| secD GENE-94 | GGATGGGATAGTTTTGTGC | ATCTATTTTGATTTGTTGAACTACC |
|
oppA
GENE-138 |
GTTACAGTTAAGAGCTTTGATG | ATTGATATAGATCGGTTGGTTC |
|
argS
GENE-183 |
CATGGCGGAGATATGATAGA | TTGCATCATTTCCAACTTCTTC |
|
hiss
GENE-236 |
CTTTTGAGTCAAGAAATAACTACAT | TTTTTTTCATCTTCATTCAAATAAGCAA |
| MHO_1160a
GENE-265 |
TTTTAGAAGATTTTATTTGCCCACA and TTTAGAAGATTTTATTTGCCCGC b | TAAAGTCGCCATTAGCCTG |
|
tyrS
GENE-428 |
CTTGCTTCAAGGTTGAGATTTTA | CTTTTGGTTTTATTTTCATTGTGTTG |
|
dnaG
GENE-519 |
CTTCCCACTTCATCCTATTTC and ACTTTCCACTTCATCCTATTTCAA b | GATTGGCCTGTTTCTTTCATTC |
a gene encoding a hypothetical protein. Gene name relates to annotation in ATCC 23114
b due to SNP in the primer footprint, equal volumes of 2 primers are required to ensure amplification of the target.