Table 1.
Mortality, mean death time, 50% infectious dose, 50% lethal dose and number of birds shedding for three H5N2 highly pathogenic avian influenza viruses in 4-week old directly inoculated and contact exposed broad-breasted white turkeys
| Isolate | Dose (EID50/bird) | Mortality | Mean death time (days) | Number of turkeys shedding | Seroconversion | 50% Turkey infectious dosee | ||||
|---|---|---|---|---|---|---|---|---|---|---|
| Inoculated | Contact exposed | Inoculated | Contact exposed | Inoculated | Contact exposed | Inoculated | Contact exposed | |||
| A/northern pintail/WA/40964/2014 | 102 | 0/5a | 0/3a | NAb | NA | 0/5d | 0/3d | 0/5i | 0/3i | 105 EID50 f |
| 104 | 0/5 | 0/3 | NA | NA | 0/5 | 0/3 | 0/5 | 0/3 | ||
| 106 | 5/5 | 3/3 | 5.3 (4–7)gh | 7.6 (7–8)cg | 5/5 | 3/3 | NA | NA | ||
| 106 | 16/16 | NA | NA | 16/16 | NA | NA | NA | |||
| A/turkey/MN/12582/2015 | 102 | 0/5 | 0/3 | NA | NA | 0/5 | 0/3 | 0/5 | 0/3 | 105 EID50 |
| 104 | 0/5 | 0/3 | NA | NA | 0/5 | 0/3 | 0/5 | 0/3 | ||
| 106 | 5/5 | 3/3 | 5.9 (3–10) | 8.0 (6–10) | 5/5 | 3/3 | NA | NA | ||
| 106 | 16/16 | NA | NA | 16/16 | NA | NA | NA | |||
| A/chicken/IA/13388/2015 | 102 | 0/5 | 0/3 | NA | NA | 0/5 | 0/3 | 0/5 | 0/3 | 103 EID50 |
| 104 | 5/5 | 3/3 | 5.4 (1–7) | 9.0 (6–10) | 3/5 | 3/3 | NA | NA | ||
| 106 | 5/5 | 3/3 | 5.6 (3–13) | 7.6 (2–13) | 5/5 | 3/3 | NA | NA | ||
| 106 | 16/16 | NA | NA | 16/16 | NA | NA | NA | |||
aNumber dead/total
b NA not applicable. Either no mortality or no birds survived for antibody testing
cCalculated from the day of placement with the inoculated turkeys
dNumber shedding/total
e50% infectious dose was equal to the 50% lethal dose for all isolates
fEID50 = 50% egg infectious dose
gMean death time days (range of mortality in days). For inoculated birds includes data from all 21 turkeys exposed to 106 EID50/bird
hMean death times are combined for directly exposed turkeys in the same dose group for each isolate
iNumber seroconverted/total