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. 2016 Nov 23;17:486. doi: 10.1186/s12891-016-1342-y

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Phase-contrast microscopy. Representative images of cells stimulated by different metals (0.1 mM). Panels a and b represent phase contrast (A1→A8) and fluorescent images (B1→B8), overlapped with counterstained nuclei. (A1-B1: untreated cells; A2-B2: Ti; A3-B3: Co; A4-B4: Mo; A5-B5: NiCl₂; A6-B6: Ni; A7-B7: CrCl₃; A8-B8: Cr-treated samples. (Phalloidin TRITC-conjugate and Hoechst 33258). (1-3; 5-7 = 40× original magnification), (4; 8 = 63× original magnification)