Table 2.
Culture Media | Shape and appearance of NTS | Motility of NTS | Duration/Viability of NTS |
---|---|---|---|
Basch Medium 169 | Parasites (NTS) appear robust and uniform | High dynamic motility | Keeps parasites alive up to one week’s incubation. Worms survive more with 10% mortality for up to 4 weeks |
DMEM | Parasites (NTS) appear distorted including rounding and darkening | Slowed and less dynamic motility | Keeps parasites alive up to 144 h When the culture media is appropriately supplemented schistosomula can be grown for at least two months |
Medium 199 | Generate different degrees of distorted parasites (NTS) including rounding and darkening | Slowed and less dynamic motility | Supplemented Medium 199 keeps the parasites alive up to 120 h with an average viability value of about 2.5 |
RPMI 1640 | Parasites (NTS) rounding | Slowed and less dynamic motility | Parasites degeneration and average death of 40 to 60% within 3 days with continued mortality up to two weeks When the culture media is appropriately supplemented schistosomula can be grown for at least two months |
NTS newly transformed schistosomula, DMEM Dulbecco’s modified eagle’s medium, RPMI Roswell Park Memorial Institute