Fig. 3.

Growth profile of E. coli LSBJ and RSC02 during PHA-biosynthesis utilizing sodium butyrate (C4) or decanoic acid (C10). For both substrates, RSC02 displayed an extended lag time and slightly slower growth rate relative to LSBJ; however, RSC02 cultures reached approximately the same density as LSBJ upon reaching the stationary phase. For both strains, growth with sodium butyrate as the substrate caused a shorter lag phase than with decanoic acid substrate. All values are the averages of triplicate experiments plus or minus the standard deviation around those averages