Figure 2.

A summary of the physiological findings of rat pyramidal cells and their relationship to mossy fiber BDNF protein expression.

Hippocampal slice physiology and immunocytochemical observations are summarized for adult male rats, either intact or orchidectomized or females at different stages of the estrous cycle and after ovariectomy. MF= mossy fiber. Evoked-response: response recorded in stratum lucidum or pyramidale of area CA3 after a stimulus to the mossy fiber pathway. Experimental procedures were standardized as described previously (Skucas et al. 2013). Epileptiform = whether mossy fiber stimulation evoked repetitive field potentials (hyperexcitability) in response to a 10 second 1 Hz train of paired pulses (Scharfman et al. 2003; Scharfman et al. 2007). BDNF effect = the physiological effect on a mossy fiber evoked-response of superfused BDNF (Scharfman 1997; Scharfman et al. 2003). BDNF protein = Relative expression of BDNF protein by immunocytochemistry (Scharfman et al. 2003; Scharfman et al. 2007). nt = not tested. The micrographs at the bottom are from previous studies (Scharfman et al. 2003) or are unpublished.