Table 1. Overview of C. difficile genotypes isolated from soil and puddles with number of isolates obtained and number of sampling sites where the genotype was found.

PCR ribotype	Toxinotype1	Puddle water2	Soil2	PCR ribotype found in
				humans	animals
002	0	7/1		+	+
005	0		10/1	+	+
010	Tox-, lok 1/3+	12/1	80/6	+	+
012	0		1/1	+	+
014/020	0	26/3	81/7	+	+
015	Tox-, lok 1/3+	11/1		+	+
023	IV (CDT+)	2/1	8/1	+	+
029	0		9/1	+	+
103	0		20/2	+	+
153(CE)	XId (CDT+)	5/1		-	-
244	IXb (CDT+)	1/1		+	+
251	IIIb (CDT+)	9/1	5/1	+	+
SLO 002	Tox-, lok 1/3+	10/1		+	+
SLO 029	0		5/1	+	+
SLO 069	0	12/2		+	+
SLO 092	Tox-, lok 1/3+		2/1	-	-
SLO 187	XId (CDT+)	9/1		+	-
SLO 191	0	5/1		+	-
SLO 192	Tox-, lok 1/3+	7/3		+	-
SLO 204	Tox-, lok 1/3-	2/1	16/5	-	-
SLO 205	Tox-, lok 1/3-		19/3	-	-
SLO 206	Tox-, lok 1/3-		9/1	-	-
SLO 208	Tox-, lok 1/3-		1/1	-	-
SLO 214	Tox-, lok 1/3-		11/1	-	-
SLO 215	Tox-, lok 1/3-		4/1	-	-
SLO 216	Tox-, lok 1/3-		8/1	-	-
SLO 218	Tox-, lok 1/3-		13/1	-	-
SLO 221	Tox-, lok 1/3-	1/1		-	-
SLO 222	Tox-, lok 1/3-		10/1	-	-
SLO 223	Tox-, lok 1/3-		12/2	-	-
SLO 229	Tox-, lok 1/3-		12/2	+	-
SLO 230	XXXII		15/1	-	-
SLO 240	Tox-, lok 1/3-		9/1	-	-
SLO 251	Tox-, lok 1/3-		1/1	-	-
Total nr. of isolates/total nr. of C. difficile positive samples		119/15	361/29	na	na
Nr. of PCR ribotypes		15	24	na	na
Nr. of tested samples		104	79	na	na

¹Tox—refers to PaLoc negative strains; lok1/3+ refers to strains where 115-bp sequence, replacing the PaLoc was PCR amplified; lok1/3—refers to PaLoc negative strains where 115-bp sequence could not be amplified with primers lok1/lok3; CDT+ indicates presence of binary toxin genes

²number of isolates/number of sampling sites; na—not applicable