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. Author manuscript; available in PMC: 2017 Nov 15.
Published in final edited form as: J Immunol. 2016 Oct 7;197(10):3871–3883. doi: 10.4049/jimmunol.1601114

Table III.

Primer sets for real-time RT-PCR

Species Gene (Gene bank no.) Direction Primer sequence
Human TACE (NM_003183) Forward CGCATTCTCAAGTCTCCACA
Reverse CACATCCCAAGCATCCTTTT
RANKL (NM_003701) Forward ATCGTTGGATCACAGCACATC
Reverse AGACTCACTTTATGGGAACCAGA
TNF-α (NM_000594) Forward ATGAGCACTGAAAGCATGATCC
Reverse GAGGGCTGATTAGAGAGAGGTC
β-actin (NM_001101) Forward CTGGAACGGTGAAGGTGACA
Reverse AAGGGACTTCCTGTAACAATGCA

Mouse Atp6v0d2 (NM_175406) Forward GAAGCTGTCAACATTGCAGA
Reverse TCACCGTGATCCTTGCAGAAT
c-Src (NM_001025395) Forward CCAGGCTGAGGAGTGGTACT
Reverse CAGCTTGCGGATCTTGTAGT
DC-STAMP (NM_029422) Forward TGGAAGTTCACTTGAAACTACGTG
Reverse CTCGGTTTCCCGTCAGCCTCTCTC
Integrin β3 (NM_016780) Forward GGCCTTCGTGGACAAGCCTG
Reverse CGGGACACCTGGTCGGTTAG
vATPase (AB022322) Forward TCCAACACAGCCTCCTACTT
Reverse ACAGCAAAGGCAGCAAAC
GAPDH (NM_008084) Forward CTCCCACTCTTCCACCTTCG
Reverse TTGCTGTAGCCGTATTCATT