Validation of proteomic data for the proteins flotillin-1, sarcosynapsin, supervillin, versican core protein and FHL1 by immunolocalization studies performed on skeletal muscle section of desminopathy patients. Abnormal muscle fibers were detected by immunostaining with primary antibodies against myotilin (B, E, H, K, N). Corresponding to the results of proteomic analysis, abnormal fibers show an increased immunoreactivity for flotillin-1 (A), sarcosynapsin (D), supervillin (G), versican core protein (J) and FHL1 (M). C, F, I, L, O: merged images. Scale bar = 25 µm.