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. 2016 Oct 26;5:e18295. doi: 10.7554/eLife.18295

Figure 6. Genetic ablation of Notch expressing cells affects the size of the lymph gland.

(A) Expression of dome-MESO-LacZ in the lymph gland 16 hr after hatching. Four cells (arrow) lacking dome-MESO-LacZ expression are seen. (B) dome- MESO-LacZ expression in third instar larval lymph gland. (C) Activation of Reaper (rpr) by N- Gal4 in a short window of 4 hr resulted in elimination of the Notch positive HSCs. (D) Rearing the larvae of above genotype to third instar stages revealed a dramatic reduction in size of the lymph gland. (E–F) Quantitative analysis of average number of cells in the lymph glands of UAS-rpr/ MESO-LacZ; tubGal80ts; N-Gal4 revealed a 5 fold reduction in comparison to wild-type (E, 2.94016E-10; n = 11, two tailed unpaired Student’s t-test). Similarly we also observed a 2.4 fold reduction in progenitor cell index (measured as the ratio of average area of Dome expressing cells with respect to total area of the lymph gland lobe) in comparison to the sibling control (F, p=1.2172E-08, two tailed unpaired Student’s t-test, n = 11). Scale bar is 20 μm in B and D and 5 μm in A and C. Genotypes are shown on top of corresponding panels. DAPI marks the nucleus. Hours after larval hatching are as indicated in each panel. Error Bars=S.D.

Figure 6—source data 1. Contains numerical data plotted in Figure 6E,F.
elife-18295-fig6-data1.xlsx (35.1KB, xlsx)
DOI: 10.7554/eLife.18295.020

Figure 6.

Figure 6—figure supplement 1. HSCs and progenitor lineages in the lymph gland.

Figure 6—figure supplement 1.

A scheme showing fate maps of progenitor and N expressing HSCs. (A) Progenitor cells give rise to all cells of the lymph gland. (B) N expressing cells are founder cells of progenitors, thus can also give rise to majority of the cells of the lymph gland through an intermediate step of progenitor generation. N expressing HSCs are higher in order to dome expressing progenitors. All cells are color coded according to G-TRACE construct (red: live; green: lineage traced; yellow: live and lineage traced). DV: dorsal vessel; Pro: progenitors; HSC: N expressing cells.