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. Author manuscript; available in PMC: 2017 Feb 11.
Published in final edited form as: Nature. 2016 Aug 11;536(7615):224–228. doi: 10.1038/nature19309

Figure 2. Cotranslational enrichment of SRP.

Figure 2

a, Srp72p-TAP was immunoprecipitated from the total soluble fraction. SRP-bound monosomes and polysomes were separated by sucrose gradient ultracentrifugation. b, Distributions of the ORF enrichment of ribosome-protected reads from SRP-bound soluble polysomes over the total soluble polysomes. ORFs were alternatively classified by expected SRP dependence11. *p ≤ 0.05, Wilcoxon rank-sum test. c, Cotranslational membrane-fraction enrichment compared to SRP enrichment. d, Metagene analysis of soluble SRP-bound polysome-protected reads from transcripts that are at least 2-fold SRP-enriched. ORFs were aligned at the targeting signal and scaled.