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. Author manuscript; available in PMC: 2017 Oct 1.
Published in final edited form as: Biochem J. 2016 Jul 26;473(19):3291–3305. doi: 10.1042/BCJ20160466

Figure 3. Wls deficiency in CD133+ DP cells inhibits matrix cells differentiation.

Figure 3

Paraffin sections of mid-dorsal skin biopsies of P32 or P35 old CD133-CreERT2; Wlsfl/fl mice and control littermates were analyzed by immunofluorescent staining to assess the expression of following markers: Gata3 for inner root sheath (control: A and C; mutant: B and D); Sox9 for outer root sheath (control: E and G; mutant: F and H); K15 for hair follicle stem cells and secondary hair germ cells (control: I and K; mutant: J and L); Ki67 for proliferating matrix cells (control: M and O; mutant: N and P); Lef1 for proliferating matrix cells and hair shaft precursor cells (control: Q and S; mutant: R and T); MITF for mature melanocytes (control: U and W; mutant: V and X). Sections were nuclear counterstained with DAPI (blue). Images shown are representative of at least three replicates at each indicated age. Scale bars: 200 µm.