SLO imaging parameters measured and/or derived for zebrafish as compared to other mammals such as humans and rodents. SLO focus span and focus translation rate measured for rats, mice and zebrafish (A). Lateral resolutions measured from a series of four different lenses with increasing refractive power (B). Lateral resolutions @ 10% MTF contrast for the four different lenses with species dependent ocular refractive powers inserted to estimate spatial resolution for humans, rats, mice, and zebrafish (C). Confocal depth of field (DOF) measured from a series of four different lenses with increasing refractive power (D). Estimated confocal DOF @ FWHM for the four different lenses shown plotted with a linear curve fit. The species dependent ocular refractive powers are shown inserted to estimate confocal DOF for humans, rats, mice, and zebrafish (E).