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. Author manuscript; available in PMC: 2018 Jan 1.
Published in final edited form as: Biomaterials. 2016 Oct 4;112:1–9. doi: 10.1016/j.biomaterials.2016.09.030

Figure 5.

Figure 5

Quantification of density and length of axons in day28 DRG single cultures in liquid phase (D-LC) and at air-liquid interface (D-ALI); DRG and hCSSCs co-culture in liquid phase(SD-LC) and at air-liquid interface (SD-ALIC); DRG co-culture with hCECs in liquid phase (ED-LC) and at air-liquid interfaces (ED-ALIC); DRG neuron, hCSSCs and hCECs tri-culture in liquid phase (ESD-LC) and at air-liquid interfaces (ESD-ALIC). The air-liquid interface culture supported significantly longer axons compared to the liquid cultures. D-ALIC and ESD-ALIC groups provided the densest axons among the groups reaching an average of ~100 termini/cm2. Data was collected from n>3 from three independent experiments. Standard deviation of each group is indicated as error bars. ***P<0.0001; **P<0.00; *P<0.01.