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. Author manuscript; available in PMC: 2018 Jan 1.
Published in final edited form as: Biomaterials. 2016 Oct 4;112:1–9. doi: 10.1016/j.biomaterials.2016.09.030

Figure 6.

Figure 6

Gene expression of hCSSCs (A) and hCECs (B). The expression levels of mRNA for involucrin (IVL), connexin 37 (GJA4) and cytokeratin 3 (KRT3) were quantified through Q-PCR and normalized with day 0 samples of hCECs. The expression levels of mRNAs for keratocan (KERA), lumican (LUM), aldehyde dehydrogenase 3A1 (ALDH3A1) and smooth muscle actin (ACTA2) were quantified through Q-PCR and normalized with day 0 samples of hCSSCs. The expression level of IVL and GJA4 were significantly higher in ED and ESD co-cultures compared to hCECs single cultures. hCSSCs expressed the most keratocan when cultured in the liquid phase. SD-ALIC groups had significantly higher keratocan expression compared to the S-ALIC groups. The data were collected from n>3 from three independent experiments. Standard error of each group is indicated as error bars. ***P<0.0001; **P<0.001; *P<0.01.